pGlo Lab

1. The first colony had about 100 colonies. It glowed orange under Uv light. The second plate had about 120 colones, but it didn't glow under Uv light. The third one didn't have any colonies.
2. The transformed bacteria now glow under a Uv light, and are resistant to ampicillin (the antibody.)
3. I think we had very few bacteria put onto the agar plates because there are a lot that could die in the process. You only need 1 to survive for there to be colonies.
4. Arabinose is able to make the E. coli survive the ampicillin and grow colonies in its presence.
5. GFF is being used in science for multiple research reasons. One use is to test whether a plasmid has successfully been inserted into another bacteria. Another use is to use it as a marker when doing tests in bacteria. One example of its use is when they inserted it into a parasite and infected another organism. They would easily be able to tell if the host was infected if it glows because of the gene.
6. One application of genetic engineering is using the E. Coli gene to cure malaria. They made the E. coli get a gene that makes it harmless and cures malaria for a much cheaper cost. This is a very important technology is third world countries stricken with malaria as the treatments that used to cost $50 are now $5.